PAT/bar
Colloidal Gold · GMO · PAT/bar
PAT/bar genes, often integrated into genetically modified crops like maize and soybeans, encode proteins that confer resistance to certain herbicides, such as glufosinate. This trait enables effective weed control, leading to increased crop yields and reduced labor and herbicide costs for farmers. Furthermore, the use of glufosinate-resistant crops allows for more diverse weed management strategies, promoting sustainable agriculture by reducing reliance on specific herbicides and minimizing environmental impact.
However, concerns regarding herbicide resistance evolution in weeds and potential non-target effects necessitate careful stewardship and diversified weed management approaches. In summary, PAT/bar in GMOs offers benefits such as enhanced weed control, cost savings, and reduced environmental impact, though cautious management is crucial to address potential risks and ensure sustainable agricultural practices.
LifeClues
PAT/bar
LifeClues test Kit for PAT/bar is designed to be used for the rapid and qualitative detection of the herbicide-resistant transgenic phosphinothricin-Nacetyltransferase protein in plants (PAT/bar).
Limit of detection: 1 ng/ml or 1 in 1000 Kernels
Ultra Sensitive
Detect target molecules at ultra-low concentrations using colloidal gold nanoparticles.
High Specificity
Engineered for precise interactions, our assays minimize false positives for accurate diagnostics.
Instant Reaction
With rapid colorimetric response, results are delivered swiftly, enabling prompt interventions.
PAT/bar RAPID TESTS QUICK GUIDE
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Preparation of the sample is a crucial step for the test to run effectively. To prepare the sample, first grind the plant seeds/ leaves/ seedlings/ other materials that need testing. Mix the sample with water according to the predetermined ratio given below:
For single seed types:
Ground a single seed sample (0.5-2 g ground seeds) in a container and add water. Mix for 15–25 seconds and let the sample settle before testing.
For a mixture of different seed types:
Grind 200 g of mixed seeds. In a beaker, combine 20-50 g of the ground sample with water. Mix for 2-5 minutes and let the sample settle before testing.
For leaf tissue from the single plant type:
Take a 1-inch x 1-inch leaf punches from the sample leaf tissue (0.15-0.3 g); push the leaf punches into the tube using a pestle. Add 3 mL of water into tube and grind the tissue using the pestle. This will result in a dilution of 1:20, if 0.15 g or 1:10, if 0.3 g. Let the paste settle for at least 45 seconds before testing or further dilution with water.
For leaf tissue from a mixture of different plant types:
Take a 1-inch x 1-inch leaf punches from each of the different sample leaf tissue (total 0.2-0.4 g); push the leaf punches into the tube using a pestle. Add 4mL of water into tube and grind the tissue using the pestle. This will result in a dilution of 1:20, if 0.2 g or 1:10, if 0.4 g. Let the paste settle for at least 45 seconds before testing or further dilution with water.
Nitrocellulose Membrane
With precise molecular immobilization capabilities, these membranes ensure accurate and reliable detection of target analytes in our colloidal gold-based assays
PAT/bar Rapid Test
INFORMATION
Test Kit Name PAT/bar Rapid Test Kit
Limit of Detection 1 ng/ml or 1 in 1000 Kernel
Test Specimens Corn seeds, Corn Leaf, Cotton seeds
Type of Service Qualitative
Test Format Lateral flow strip
