CP4 EPSPS
Colloidal Gold · GMO · CP4 EPSPS
CP4 EPSPS, an enzyme from Agrobacterium tumefaciens, is often inserted into GMOs like soybeans and maize to confer resistance to glyphosate-based herbicides. While this trait reduces herbicide costs and simplifies weed management, it poses risks. Overreliance on glyphosate can lead to the emergence of herbicide-resistant weeds, requiring more potent herbicides. Moreover, glyphosate's classification as a probable carcinogen raises health and environmental concerns. Its widespread use may harm non-target plants and disrupt ecosystems. In summary, while CP4 EPSPS enhances crop resilience, its hazards, including herbicide resistance and environmental impacts, necessitate careful management and ongoing research.
LifeClues
CP4 EPSPS
The LifeClues Rapid test Kit for CP4 EPSPS is intended to test the presence of CP4 EPSPS protein at a level typically expressed in genetically modified (GM) Roundup Ready Crop (Soybeans, Corn, Cotton, etc) to assist the determination of Roundup Ready Crops.
Limit of detection: 1 ng/ml, or 0.1%
(i.e. one Roundup Ready seed in 1000 conventional kernels)
Ultra Sensitive
Detect target molecules at ultra-low concentrations using colloidal gold nanoparticles.
High Specificity
Engineered for precise interactions, our assays minimize false positives for accurate diagnostics.
Instant Reaction
With rapid colorimetric response, results are delivered swiftly, enabling prompt interventions.
CP4 EPSPS RAPID TESTS QUICK GUIDE
Determine Number and Size of Sub-samples
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For sampling plan, please read literatures in the Reference Sections. Collect a composite sample according to USDA/ GIPSA instructions found in the reference literature.
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To select an appropriate sample size, determine the purity standard and the degree of confidence required. Confidence level means the statistical probability that the true Roundup Ready level in the lot is below the selected purity standard. Table 1 provides a guideline for determining the number of beans in each sub-sample that are necessary to provide effective screening for different GM concentrations at the 95% and 99% confidence levels.
Determine Sub-Sample Weight, and Grind Times
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Determine average weight of individual grain to be tested (weigh 100 beans divide by 100).
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Calculate the weight of the number of grains to be tested (Number of grains x Average Weight/Grain). Table 2 lists the guidelines for grinding time according to sample weight.
Determine the Volume of Water to be Used in Extraction
The test is suitable for testing both leaf tissue and seed of Soybean, corn, cotton and other crops.Leaves, or seeds must be grinded and diluted with water (Tab water could be used). For best result, different samples should be diluted with water in different ratio (see table below). Let the paste set for at least 45 seconds before testing with the test kits.
Nitrocellulose Membrane
With precise molecular immobilization capabilities, these membranes ensure accurate and reliable detection of target analytes in our colloidal gold-based assays
CP4 EPSPS Rapid Test
INFORMATION
Test Kit Name CP4 EPSPS Rapid Test Kit
Limit of Detection 1 ng/ml, or 0.1% (i.e. one Roundup Ready seed in 1000
conventional kernels)
Test Specimens Extract of Soybean, Corn, Cotton and other Crops
Type of Service Qualitative
Test Format Lateral flow strip
